Depends on the Company Rules and the Special Instructions. Variabilities include cars with in-operable brakes, per-cent of the grade, curvature, distance for retainer use, weight of the train (remember Tons Per Operable Brake?), dynamic abilities of the locomotives among some of the considerations.
And, just to add a little confusion for you, although the retainers would normally be turned up at the top of a grade, that may not be the case. It has been known for crews to be required to use retainers going up a grade as well as down (really rare), and if a crew is waiting for another train someplace over the other side of the retainer district, they can turn up the retainers there, but if they do and they need to use the brakes prior to the summit, the brakes on the cars that have been turned up will stay applied and probably stall the train.
AND … If the lead unit of the train has its brake system incorporating the pressure maintaining function (all units built new in the last 25 or so years – at least --) may not be required to use retainers. As I remember, Saluda is pretty short and I think that a stop for wheel radiation or resetting of the retainers would not be required. All that would be required in this case is a “blow and go” or a running test.